Review




Structured Review

Merck & Co cryostor cs10
Cryostor Cs10, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cryostor+cs10/cryostor+cs10/pm41480010-73-8-11
Average 86 stars, based on 1 article reviews
cryostor cs10 - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Produced:

Article Title: Preclinical assessment of MAGE-A4-specific TCR-NK cells against solid tumors
Article Snippet: The cells were expanded, and the transduced population was positively selected using CD3 Microbeads (130-050-101, Miltenyi Biotec). .. The selected cells were pulsed with feeder cells (produced in house), harvested on Day 21–28, cryopreserved in Cryostor CS10 (C2874-100ML, Merck Sigma Aldrich) with 2.5% HSA (05-730-1E, Sartorius), or in Plasmalyte (AFE0324D, Baxter)/HSA/Cryostor CS10 (3:1:4 ratio) and stored in liquid N 2 until use. .. The TCR-NK cells were thawed and cultured in CTS medium with supplement (A5019001, GIBCO Thermo Fisher Scientific), containing 5% human AB serum (535-HI-GI, Grifols), IL-2, and IL-15 (170-076-146, 170-076-114, Miltenyi Biotec) for 72 h before use in the functional assays.

Article Title: Preclinical assessment of MAGE-A4-specific TCR-NK cells against solid tumors.
Article Snippet: The cells were expanded, and the transduced population 4 was positively selected using CD3 Microbeads (130-050-101, Miltenyi Biotec). .. The selected cells 5 were pulsed with feeder cells (produced in house), harvested on day 21-28, cryopreserved in 6 Cryostor CS10 (C2874-100ML, Merck Sigma Aldrich) with 2.5% HSA (05-730-1E, Sartorius), or in 7 Plasmalyte (AFE0324D, Baxter) /HSA/Cryostor CS10 (3:1:4 ratio) and stored in liquid N2 until use. .. 8 The TCR-NK cells were thawed and cultured in CTS medium with supplement (A5019001, GIBCO 9 Thermo Fisher Scientific), containing 5% human AB serum (535-HI-GI, Grifols), IL-2, and IL-15 10 (170-076-146, 170-076-114, Miltenyi Biotec). for 72 hours before use in the functional assays.

Isolation:

Article Title: Preclinical assessment of MAGE-A4-specific TCR-NK cells against solid tumors
Article Snippet: .. After collection, peripheral blood mononuclear cells (PBMCs) were isolated through density gradient centrifugation using SepMate tubes (StemCell Technologies) and Lymphoprep (47421, ProteoGenix), cryopreserved in Cryostor CS10 (C2874-100ML, Merck Sigma Aldrich) and stored in liquid N 2 until use. .. Frozen leukapheresis were purchased from StemCell Technologies (200-0132) and AllCells (LP CR Solo 2.5-3.0B) and were stored at −150°C until use.

Gradient Centrifugation:

Article Title: Preclinical assessment of MAGE-A4-specific TCR-NK cells against solid tumors
Article Snippet: .. After collection, peripheral blood mononuclear cells (PBMCs) were isolated through density gradient centrifugation using SepMate tubes (StemCell Technologies) and Lymphoprep (47421, ProteoGenix), cryopreserved in Cryostor CS10 (C2874-100ML, Merck Sigma Aldrich) and stored in liquid N 2 until use. .. Frozen leukapheresis were purchased from StemCell Technologies (200-0132) and AllCells (LP CR Solo 2.5-3.0B) and were stored at −150°C until use.



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